Properties of phospholipase C isolated from rat liver lysosomes.
نویسندگان
چکیده
منابع مشابه
Isolating Lysosomes from Rat Liver.
This protocol describes the generation of a fraction enriched in lysosomes from rat liver. The lysosomes are rapidly isolated using density-gradient centrifugation with gradient media that retain the osmolarity of the lysosomes such that they are functional and can be used in in vitro assays.
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The interactions of tributyllead with lysosomes from rat liver have been studied. It results that the organometal compound induces a fast alkalinization in energized lysosomes. The interpretation is that the compound is a potent proton carrier. This function could explain the toxicity, in particular at neurological level of the compound.
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The subcellular distribution in rat liver of a phospholipid identified as bis-(monoacylglyceryl) phosphate (bis(MAG)P) has been investigated. In liver from untreated animals, 48 % of bis(MAG)P was recovered in a crude mitochondrial fraction where it was enriched 2.8-fold over the homogenate in terms of protein. When liver from animals who had received Triton WR-1339 by injection 3 days earlier ...
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The phosphatases of rat liver lysosomes have been fractionated chromatographically. One of these enzymes has been characterized as a nucleotidase of broad specificity, acting equally well on most 2’-, 3’-, and S’nucleotides. Evidence is presented regarding its substrate specificity, stability, and pH optimum, and the effects of inorganic and organic compounds on its activity are presented. Sucr...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)86225-7